en فيزيولوژی ورزشی و شاخه‌های وابسته بدان Sport Physiology and its related branches مقاله اصیل Original Article <div style="text-align: justify;"><strong>Background. </strong>Curcumin supports metabolism with its antioxidant and anti-inflammatory effects. It is particularly effective in reducing metabolic stress caused by prolonged exercise.<br> <strong>Objectives. </strong>This study aimed to investigate the impact of curcumin supplementation on Irisin, Nesfatin-1, and Leptin hormone levels in rats undergoing prolonged treadmill exercise.<br> <strong>Methods. </strong>A total of 32 male Wistar-Albino rats were divided randomly into four equal groups: The control group (n=8), the curcumin group (n=8), the treadmill group (n=8), and the curcumin+treadmill group. The experiment lasted eight weeks, with one week of treadmill acclimatization. No intervention was applied to the control group. Curcumin was given at 200 mg/kg, and treadmill exercise was performed for 30 min at 45 cm/s, three days weekly. The Curcumin+Treadmill group was fed 200 mg/kg/day and performed three days a week for 30 minutes at an average speed of 45 cm/s. After the study, rats were euthanized, and blood samples were collected for analysis. Irisin, nesfatin-1, and leptin hormone levels were determined using the ELISA method.<br> <strong>Results.</strong> Our study showed a significant increase in irisin levels in both the curcumin and exercise groups compared to the control group. For nesfatin-1, levels were significantly decreased in the curcumin group compared to the control, while they were significantly increased in the curcumin+exercise group. Leptin levels were the highest in the exercise group, showing a significant increase compared to the control group (p<0.05). In contrast, the leptin levels in the curcumin group were significantly lower than those in the exercise group.<br> <strong>Conclusion. </strong>This study demonstrates that combining curcumin supplementation and exercise positively affects irisin, nesfatin-1, and leptin hormone levels, contributing to metabolic balance.</div> Exercise, Supplementation, Curcumin, Leptin, Irisin, Nesfatin-1. 0 0 http://aassjournal.com/browse.php?a_code=A-11-2335-5&slc_lang=en&sid=1 Aysun Delioglan aysundelioglan3@gmail.com 100319475328460017056 0009-0004-2845-3324 No Gaziantep University, Department of Physical Education and Sport, Gaziantep, Turkey Zarife Pancar z_pancar@hotmail.com 100319475328460017057 100319475328460017057 Yes Gaziantep University, Faculty of Sports Science, Department of Physical Education and Sports Gaziantep, Turkey Muhammed Kaan Darendeli kaandrndl@hotmail.com 100319475328460017058 100319475328460017058 No Gaziantep University, Department of Physical Education and Sport, Gaziantep, Turkey Yesim Kılıcoğlu yesimkilicoglu8888@gmail.com 100319475328460017059 0009-0008-2300-6639 No Gaziantep University, Department of Physical Education and Sport, Gaziantep, Turkey Hasan Ulusal hasan_ulusal@hotmail.com 100319475328460017060 100319475328460017060 No Gaziantep University, Department of Medical Biochemistry, Gaziantep, Turkey Mufit Dal mufitdal1977@gmail.com 100319475328460017061 100319475328460017061 No Gaziantep University, Department of Physical Education and Sport, Gaziantep, Turkey Ali Muhittin Tasdogan drmtasdogan@gmail.com 100319475328460017062 100319475328460017062 No Hasan Kalyoncu University, Faculty of Health Sciences, Gaziantep, Turkey Xu Yan xu.yan@vu.edu.au 100319475328460017063 100319475328460017063 No Victoria University, Insitute for Health and Sport, Melbourne, Australia